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Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
Here, we present a protocol for harnessing the natural transformability of the edible algae Arthrospira platensis (common name: spirulina) to generate strains that express heterologous proteins. We describe the preparation of plasmids and the steps to grow A. platensis. We then detail the transforma...
Autores principales: | Tabakh, Hannah, Jester, Benjamin W., Zhao, Hui, Kuestner, Rolf, Khuong, Nhi, Shanitta, Chelsea, Takeuchi, Ryo, Roberts, James |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929480/ https://www.ncbi.nlm.nih.gov/pubmed/36853691 http://dx.doi.org/10.1016/j.xpro.2023.102087 |
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