Protocol to study in vitro drug metabolism and identify montelukast metabolites from purified enzymes and primary cell cultures by mass spectrometry

We present an optimized protocol set to study the production of drug metabolites in different in vitro systems. We detail the necessary steps to identify the metabolites of xenobiotics produced in different metabolic-competent systems, from purified enzymes to primary cell cultures. It is coupled to...

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Detalles Bibliográficos
Autores principales: Marques, Cátia F., Pinheiro, Pedro F., Justino, Gonçalo C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929484/
https://www.ncbi.nlm.nih.gov/pubmed/36853690
http://dx.doi.org/10.1016/j.xpro.2023.102086

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929484/
https://www.ncbi.nlm.nih.gov/pubmed/36853690
http://dx.doi.org/10.1016/j.xpro.2023.102086

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