A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites

Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cell...

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Detalles Bibliográficos
Autores principales: Pihlajamaa, Päivi, Kauko, Otto, Sahu, Biswajyoti, Kivioja, Teemu, Taipale, Jussi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931575/
https://www.ncbi.nlm.nih.gov/pubmed/36163549
http://dx.doi.org/10.1038/s41587-022-01444-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931575/
https://www.ncbi.nlm.nih.gov/pubmed/36163549
http://dx.doi.org/10.1038/s41587-022-01444-6

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