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Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae
The budding yeast Saccharomyces cerevisiae is an excellent model for examining the effects of ploidy. Here, we provide a protocol for producing polyploid cells by creating a basic unit (matΔ) and polyploidizing it via repeated mating. We describe steps for basic unit construction by one-step transfo...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932207/ https://www.ncbi.nlm.nih.gov/pubmed/36825809 http://dx.doi.org/10.1016/j.xpro.2023.102085 |