Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae

The budding yeast Saccharomyces cerevisiae is an excellent model for examining the effects of ploidy. Here, we provide a protocol for producing polyploid cells by creating a basic unit (matΔ) and polyploidizing it via repeated mating. We describe steps for basic unit construction by one-step transfo...

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Autores principales: Oya, Kazumasa, Matsuura, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932207/
https://www.ncbi.nlm.nih.gov/pubmed/36825809
http://dx.doi.org/10.1016/j.xpro.2023.102085
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author Oya, Kazumasa
Matsuura, Akira
author_facet Oya, Kazumasa
Matsuura, Akira
author_sort Oya, Kazumasa
collection PubMed
description The budding yeast Saccharomyces cerevisiae is an excellent model for examining the effects of ploidy. Here, we provide a protocol for producing polyploid cells by creating a basic unit (matΔ) and polyploidizing it via repeated mating. We describe steps for basic unit construction by one-step transformation, increased ploidy via repeated mating, and ploidy confirmation using flow cytometry. This protocol can be broadly applied to evaluate the physiology of polyploid cells. For complete details on the use and execution of this protocol, please refer to Oya and Matsuura (2022).(1)
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spelling pubmed-99322072023-02-17 Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae Oya, Kazumasa Matsuura, Akira STAR Protoc Protocol The budding yeast Saccharomyces cerevisiae is an excellent model for examining the effects of ploidy. Here, we provide a protocol for producing polyploid cells by creating a basic unit (matΔ) and polyploidizing it via repeated mating. We describe steps for basic unit construction by one-step transformation, increased ploidy via repeated mating, and ploidy confirmation using flow cytometry. This protocol can be broadly applied to evaluate the physiology of polyploid cells. For complete details on the use and execution of this protocol, please refer to Oya and Matsuura (2022).(1) Elsevier 2023-02-02 /pmc/articles/PMC9932207/ /pubmed/36825809 http://dx.doi.org/10.1016/j.xpro.2023.102085 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Oya, Kazumasa
Matsuura, Akira
Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae
title Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae
title_full Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae
title_fullStr Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae
title_full_unstemmed Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae
title_short Artificial control of mating type and repeated mating to produce polyploid cells in Saccharomyces cerevisiae
title_sort artificial control of mating type and repeated mating to produce polyploid cells in saccharomyces cerevisiae
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9932207/
https://www.ncbi.nlm.nih.gov/pubmed/36825809
http://dx.doi.org/10.1016/j.xpro.2023.102085
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