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Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation

Complete enzymatic digestion of proteins for bottom-up proteomics is substantially improved by use of detergents for denaturation and solubilization. Detergents however, are incompatible with many proteases and highly detrimental to LC-MS/MS. Recently; filter-based methods have seen wide use due to...

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Detalles Bibliográficos
Autores principales: Mousseau, C. Bruce, Pierre, Camille A., Hu, Daniel D., Champion, Matthew M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933840/
https://www.ncbi.nlm.nih.gov/pubmed/36373982
http://dx.doi.org/10.1039/d2ay01549h