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Reliability of species detection in 16S microbiome analysis: Comparison of five widely used pipelines and recommendations for a more standardized approach

The use of NGS-based testing of the bacterial microbiota is often impeded by inconsistent or non-reproducible results, especially when applying different analysis pipelines and reference databases. We investigated five frequently used software packages by submitting the same monobacterial datasets t...

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Detalles Bibliográficos
Autores principales: Hiergeist, Andreas, Ruelle, Jean, Emler, Stefan, Gessner, André
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934417/
https://www.ncbi.nlm.nih.gov/pubmed/36795699
http://dx.doi.org/10.1371/journal.pone.0280870