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IVT generation of guideRNAs for Cas9-enrichment Nanopore Sequencing

Generating high-coverage sequencing coverage at select genomic loci has extensive applications in both research science and genetic medicine. Long-read sequencing technologies (e.g. nanopore sequencing) have expanded our ability to generate sequencing data in regions (e.g. repetitive elements) that...

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Detalles Bibliográficos
Autores principales: Gilpatrick, Timothy, Wang, Josh Zhiyong, Weiss, David, Norris, Alexis L, Eshleman, James, Timp, Winston
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934585/
https://www.ncbi.nlm.nih.gov/pubmed/36798399
http://dx.doi.org/10.1101/2023.02.07.527484