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RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids

Pigs (Sus scrofa) are an animal model for metabolic diseases in humans. Pork is an important source of fatty acids (FAs) in the human diet, as it is one of the most consumed meats worldwide. The effects of dietary inclusion of oils such as canola, fish, and soybean oils on pig gene expression are mo...

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Autores principales: Fanalli, Simara Larissa, da Silva, Bruna Pereira Martins, Gomes, Julia Dezen, Durval, Mariah Castro, de Almeida, Vivian Vezzoni, Moreira, Gabriel Costa Monteiro, Silva-Vignato, Bárbara, Afonso, Juliana, Freitas, Felipe André Oliveira, Reecy, James Mark, Koltes, James Eugene, Koltes, Dawn, Garrick, Dorian, Correia de Almeida Regitano, Luciana, Balieiro, Júlio Cesar de Carvalho, Mourão, Gerson Barreto, Coutinho, Luiz Lehmann, Fukumasu, Heidge, de Alencar, Severino Matias, Luchiari Filho, Albino, Cesar, Aline Silva Mello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9936315/
https://www.ncbi.nlm.nih.gov/pubmed/36816031
http://dx.doi.org/10.3389/fgene.2023.1053021
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author Fanalli, Simara Larissa
da Silva, Bruna Pereira Martins
Gomes, Julia Dezen
Durval, Mariah Castro
de Almeida, Vivian Vezzoni
Moreira, Gabriel Costa Monteiro
Silva-Vignato, Bárbara
Afonso, Juliana
Freitas, Felipe André Oliveira
Reecy, James Mark
Koltes, James Eugene
Koltes, Dawn
Garrick, Dorian
Correia de Almeida Regitano, Luciana
Balieiro, Júlio Cesar de Carvalho
Mourão, Gerson Barreto
Coutinho, Luiz Lehmann
Fukumasu, Heidge
de Alencar, Severino Matias
Luchiari Filho, Albino
Cesar, Aline Silva Mello
author_facet Fanalli, Simara Larissa
da Silva, Bruna Pereira Martins
Gomes, Julia Dezen
Durval, Mariah Castro
de Almeida, Vivian Vezzoni
Moreira, Gabriel Costa Monteiro
Silva-Vignato, Bárbara
Afonso, Juliana
Freitas, Felipe André Oliveira
Reecy, James Mark
Koltes, James Eugene
Koltes, Dawn
Garrick, Dorian
Correia de Almeida Regitano, Luciana
Balieiro, Júlio Cesar de Carvalho
Mourão, Gerson Barreto
Coutinho, Luiz Lehmann
Fukumasu, Heidge
de Alencar, Severino Matias
Luchiari Filho, Albino
Cesar, Aline Silva Mello
author_sort Fanalli, Simara Larissa
collection PubMed
description Pigs (Sus scrofa) are an animal model for metabolic diseases in humans. Pork is an important source of fatty acids (FAs) in the human diet, as it is one of the most consumed meats worldwide. The effects of dietary inclusion of oils such as canola, fish, and soybean oils on pig gene expression are mostly unknown. Our objective was to evaluate FA composition, identify changes in gene expression in the liver of male pigs fed diets enriched with different FA profiles, and identify impacted metabolic pathways and gene networks to enlighten the biological mechanisms’ variation. Large White male pigs were randomly allocated to one of three diets with 18 pigs in each; all diets comprised a base of corn and soybean meal to which either 3% of soybean oil (SOY), 3% canola oil (CO), or 3% fish oil (FO) was added for a 98-day trial during the growing and finishing phases. RNA sequencing was performed on the liver samples of each animal by Illumina technology for differential gene expression analyses, using the R package DESeq2. The diets modified the FA profile, mainly in relation to polyunsaturated and saturated FAs. Comparing SOY vs. FO, 143 differentially expressed genes (DEGs) were identified as being associated with metabolism, metabolic and neurodegenerative disease pathways, inflammatory processes, and immune response networks. Comparing CO vs. SOY, 148 DEGs were identified, with pathways related to FA oxidation, regulation of lipid metabolism, and metabolic and neurodegenerative diseases. Our results help explain the behavior of genes with differential expression in metabolic pathways resulting from feeding different types of oils in pig diets.
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spelling pubmed-99363152023-02-18 RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids Fanalli, Simara Larissa da Silva, Bruna Pereira Martins Gomes, Julia Dezen Durval, Mariah Castro de Almeida, Vivian Vezzoni Moreira, Gabriel Costa Monteiro Silva-Vignato, Bárbara Afonso, Juliana Freitas, Felipe André Oliveira Reecy, James Mark Koltes, James Eugene Koltes, Dawn Garrick, Dorian Correia de Almeida Regitano, Luciana Balieiro, Júlio Cesar de Carvalho Mourão, Gerson Barreto Coutinho, Luiz Lehmann Fukumasu, Heidge de Alencar, Severino Matias Luchiari Filho, Albino Cesar, Aline Silva Mello Front Genet Genetics Pigs (Sus scrofa) are an animal model for metabolic diseases in humans. Pork is an important source of fatty acids (FAs) in the human diet, as it is one of the most consumed meats worldwide. The effects of dietary inclusion of oils such as canola, fish, and soybean oils on pig gene expression are mostly unknown. Our objective was to evaluate FA composition, identify changes in gene expression in the liver of male pigs fed diets enriched with different FA profiles, and identify impacted metabolic pathways and gene networks to enlighten the biological mechanisms’ variation. Large White male pigs were randomly allocated to one of three diets with 18 pigs in each; all diets comprised a base of corn and soybean meal to which either 3% of soybean oil (SOY), 3% canola oil (CO), or 3% fish oil (FO) was added for a 98-day trial during the growing and finishing phases. RNA sequencing was performed on the liver samples of each animal by Illumina technology for differential gene expression analyses, using the R package DESeq2. The diets modified the FA profile, mainly in relation to polyunsaturated and saturated FAs. Comparing SOY vs. FO, 143 differentially expressed genes (DEGs) were identified as being associated with metabolism, metabolic and neurodegenerative disease pathways, inflammatory processes, and immune response networks. Comparing CO vs. SOY, 148 DEGs were identified, with pathways related to FA oxidation, regulation of lipid metabolism, and metabolic and neurodegenerative diseases. Our results help explain the behavior of genes with differential expression in metabolic pathways resulting from feeding different types of oils in pig diets. Frontiers Media S.A. 2023-01-25 /pmc/articles/PMC9936315/ /pubmed/36816031 http://dx.doi.org/10.3389/fgene.2023.1053021 Text en Copyright © 2023 Fanalli, Silva, Gomes, Durval, Almeida, Moreira, Silva-Vignato, Afonso, Freitas, Reecy, Koltes, Koltes, Garrick, Correia de Almeida Regitano, Balieiro, Mourão, Coutinho, Fukumasu, Alencar, Luchiari Filho and Cesar. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Fanalli, Simara Larissa
da Silva, Bruna Pereira Martins
Gomes, Julia Dezen
Durval, Mariah Castro
de Almeida, Vivian Vezzoni
Moreira, Gabriel Costa Monteiro
Silva-Vignato, Bárbara
Afonso, Juliana
Freitas, Felipe André Oliveira
Reecy, James Mark
Koltes, James Eugene
Koltes, Dawn
Garrick, Dorian
Correia de Almeida Regitano, Luciana
Balieiro, Júlio Cesar de Carvalho
Mourão, Gerson Barreto
Coutinho, Luiz Lehmann
Fukumasu, Heidge
de Alencar, Severino Matias
Luchiari Filho, Albino
Cesar, Aline Silva Mello
RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids
title RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids
title_full RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids
title_fullStr RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids
title_full_unstemmed RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids
title_short RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids
title_sort rna-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9936315/
https://www.ncbi.nlm.nih.gov/pubmed/36816031
http://dx.doi.org/10.3389/fgene.2023.1053021
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