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Monitoring cell fate in 3D organotypic human squamous epithelial cultures

Here, we provide a protocol to model the effects of changes to a small number of cells, such as those arising from a mutation or a virus infection, in stratified epithelia. We describe steps for diluting engineered human keratinocytes into a larger population of unmodified cells and using these cell...

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Detalles Bibliográficos
Autores principales: Hatterschide, Joshua, Natale, Christopher A., Ridky, Todd W., White, Elizabeth A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937944/
https://www.ncbi.nlm.nih.gov/pubmed/36853703
http://dx.doi.org/10.1016/j.xpro.2023.102101