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Monitoring cell fate in 3D organotypic human squamous epithelial cultures
Here, we provide a protocol to model the effects of changes to a small number of cells, such as those arising from a mutation or a virus infection, in stratified epithelia. We describe steps for diluting engineered human keratinocytes into a larger population of unmodified cells and using these cell...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937944/ https://www.ncbi.nlm.nih.gov/pubmed/36853703 http://dx.doi.org/10.1016/j.xpro.2023.102101 |