Chromatin immunoprecipitation with mouse adipocytes using hypotonic buffer to enrich nuclear fraction before fixation

Chromatin immunoprecipitation (ChIP) experiments with differentiated adipocytes are challenging because lipid droplets interfere with immunoprecipitation efficiency. Here, the author describes optimized procedures to minimize the burden of lipid droplets by using hypotonic buffer to enrich nuclear f...

Descripción completa

Detalles Bibliográficos
Autor principal: Hiraike, Yuta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937945/
https://www.ncbi.nlm.nih.gov/pubmed/36825994
http://dx.doi.org/10.1016/j.xpro.2023.102093

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937945/
https://www.ncbi.nlm.nih.gov/pubmed/36825994
http://dx.doi.org/10.1016/j.xpro.2023.102093

Ejemplares similares