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Protocol for multi-modal single-cell RNA sequencing on M. tuberculosis-infected mouse lungs

To elucidate how different immune cells contribute to control or progression of M. tuberculosis (Mtb) infection, we developed a technique to perform multi-modal single-cell RNA sequencing (scRNA-seq) from in vivo Mtb-infected lung macrophages. This protocol simultaneously acquires the transcriptome,...

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Detalles Bibliográficos
Autores principales: Pisu, Davide, Russell, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937979/
https://www.ncbi.nlm.nih.gov/pubmed/36853694
http://dx.doi.org/10.1016/j.xpro.2023.102102