Optimization of the CRISPR/Cas9 system using adh1 promoter derivatives in fission yeast

The CRSIPR/Cas9 system has been applied to fission yeast, but there remain some rooms for improvement. Here we report that the weaker versions of the adh1 (+) promoter, adh11 and adh41 promoters, for the potentially cytotoxic Cas9 achieved highly efficient mutagenesis and gene deletion at the ade6 (...

Descripción completa

Detalles Bibliográficos
Autores principales: Saito, Miori, Nakaoka, Hidenori, Hayashi, Aki, Takaku, Hiroaki, Yamazaki, Harutake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938407/
https://www.ncbi.nlm.nih.gov/pubmed/36820392
http://dx.doi.org/10.17912/micropub.biology.000757

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938407/
https://www.ncbi.nlm.nih.gov/pubmed/36820392
http://dx.doi.org/10.17912/micropub.biology.000757

Ejemplares similares