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An optimized and validated protocol for the purification of PDGFRα+ oligodendrocyte precursor cells from mouse brain tissue via immunopanning

Immunopanning is an efficient and reliable method for isolating primary cells from rodent brain tissue, making it a valuable tool for researchers interested in in vitro • The protocol presented here is optimized for a PDGFRα-dependent selection of mouse OPCs using a commercial antibody, accounting f...

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Detalles Bibliográficos
Autores principales: Macintosh, Julia, Michell-Robinson, Mackenzie A., Chen, Xiaoru, Chitsaz, Daryan, Kennedy, Timothy E., Bernard, Geneviève
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939712/
https://www.ncbi.nlm.nih.gov/pubmed/36814689
http://dx.doi.org/10.1016/j.mex.2023.102051