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An optimized and validated protocol for the purification of PDGFRα+ oligodendrocyte precursor cells from mouse brain tissue via immunopanning
Immunopanning is an efficient and reliable method for isolating primary cells from rodent brain tissue, making it a valuable tool for researchers interested in in vitro • The protocol presented here is optimized for a PDGFRα-dependent selection of mouse OPCs using a commercial antibody, accounting f...
Autores principales: | Macintosh, Julia, Michell-Robinson, Mackenzie A., Chen, Xiaoru, Chitsaz, Daryan, Kennedy, Timothy E., Bernard, Geneviève |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939712/ https://www.ncbi.nlm.nih.gov/pubmed/36814689 http://dx.doi.org/10.1016/j.mex.2023.102051 |
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