Cargando…

Imaging-Based In Situ Analysis of 5-Methylcytosine at Low Repetitive Single Gene Loci with Transcription-Activator-Like Effector Probes

[Image: see text] Transcription-activator-like effectors (TALEs) are programmable DNA binding proteins that can be used for sequence-specific, imaging-based analysis of cellular 5-methylcytosine. However, this has so far been limited to highly repetitive satellite DNA. To expand this approach to the...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jung, Anne, Munõz-López, Álvaro, Buchmuller, Benjamin C., Banerjee, Sudakshina, Summerer, Daniel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942090/ https://www.ncbi.nlm.nih.gov/pubmed/36693632 http://dx.doi.org/10.1021/acschembio.2c00857

Ejemplares similares

Engineered TALE Repeats for Enhanced Imaging‐Based Analysis of Cellular 5‐Methylcytosine
por: Muñoz‐López, Álvaro, et al.
Publicado: (2020)

Designer Receptors for Nucleotide‐Resolution Analysis of Genomic 5‐Methylcytosine by Cellular Imaging
por: Muñoz‐López, Álvaro, et al.
Publicado: (2020)

Overcoming conservation in TALE–DNA interactions: a minimal repeat scaffold enables selective recognition of an oxidized 5-methylcytosine
por: Maurer, Sara, et al.
Publicado: (2018)

Evolved DNA Duplex Readers for Strand-Asymmetrically Modified 5-Hydroxymethylcytosine/5-Methylcytosine CpG Dyads
por: Buchmuller, Benjamin C., et al.
Publicado: (2022)

Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition
por: Zhang, Yuan, et al.
Publicado: (2017)

Engineering DNA Backbone Interactions Results in TALE Scaffolds with Enhanced 5-Methylcytosine Selectivity
por: Rathi, Preeti, et al.
Publicado: (2017)

Advances in mRNA 5-methylcytosine modifications: Detection, effectors, biological functions, and clinical relevance
por: Guo, Gangqiang, et al.
Publicado: (2021)

Optochemical Control of TET Dioxygenases Enables Kinetic Insights into the Domain-Dependent Interplay of TET1 and MBD1 while Oxidizing and Reading 5-Methylcytosine
por: Lin, Tzu-Chen, et al.
Publicado: (2022)

Light‐Activation of DNA‐Methyltransferases
por: Wolffgramm, Jan, et al.
Publicado: (2021)

Epigenetic CpG duplex marks probed by an evolved DNA reader via a well-tempered conformational plasticity
por: Singh, Himanshu, et al.
Publicado: (2023)

Site- and degree-specific C–H oxidation on 5-methylcytosine homologues for probing active DNA demethylation
por: Kavoosi, Sam, et al.
Publicado: (2019)

Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA
por: Hashimoto, Hideharu, et al.
Publicado: (2013)

TET‐Like Oxidation in 5‐Methylcytosine and Derivatives: A Computational and Experimental Study
por: Jonasson, Niko S. W., et al.
Publicado: (2021)

Tigerfish designs oligonucleotide-based in situ hybridization probes targeting intervals of highly repetitive DNA at the scale of genomes
por: Aguilar, Robin, et al.
Publicado: (2023)

AF9 promotes hESC neural differentiation through recruiting TET2 to neurodevelopmental gene loci for methylcytosine hydroxylation
por: Qiao, Yunbo, et al.
Publicado: (2015)

Characterizing 5-methylcytosine in the mammalian epitranscriptome
por: Hussain, Shobbir, et al.
Publicado: (2013)

DNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B
por: Fu, Yang, et al.
Publicado: (2015)

Evolution of Transcription Activator-Like Effectors in Xanthomonas oryzae
por: Erkes, Annett, et al.
Publicado: (2017)

Binding of the transcription activator-like effector augments transcriptional regulation by another transcription factor
por: Leben, Katja, et al.
Publicado: (2022)

Effects of Tet-mediated Oxidation Products of 5-Methylcytosine on DNA Transcription in vitro and in Mammalian Cells
por: You, Changjun, et al.
Publicado: (2014)

Deamination of 5-Methylcytosine Residues in Mammalian Cells
por: Gromenko, E.V., et al.
Publicado: (2009)

Discovery of a Bacterial 5-Methylcytosine Deaminase
por: Hitchcock, Daniel S., et al.
Publicado: (2014)

Determination of the presence of 5-methylcytosine in Paramecium tetraurelia
por: Singh, Aditi, et al.
Publicado: (2018)

Transcription Activator-like Effectors: A Toolkit for Synthetic Biology
por: Moore, Richard, et al.
Publicado: (2014)

Comprehensive analysis of the specificity of transcription activator-like effector nucleases
por: Juillerat, Alexandre, et al.
Publicado: (2014)

Assembly and Validation of Versatile Transcription Activator-Like Effector Libraries
por: Li, Yi, et al.
Publicado: (2014)

Repetition effects in action planning reflect effector- but not hemisphere-specific coding
por: Seegelke, Christian, et al.
Publicado: (2021)

A high-throughput effector screen identifies a novel small molecule scaffold for inhibition of ten-eleven translocation dioxygenase 2
por: Palei, Shubhendu, et al.
Publicado: (2022)

Complete Profiling of Methyl-CpG-Binding Domains for Combinations of Cytosine Modifications at CpG Dinucleotides Reveals Differential Read-out in Normal and Rett-Associated States
por: Buchmuller, Benjamin C., et al.
Publicado: (2020)

Comparison of DNA methylation levels of repetitive loci during bovine development
por: Kaneda, Masahiro, et al.
Publicado: (2011)

Dual DNA binding mode of a turn-on red fluorescent probe thiazole coumarin
por: Ganguly, Sudakshina, et al.
Publicado: (2020)

Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes
por: Ni, Yanxiang, et al.
Publicado: (2017)

Locked and proteolysis-based transcription activator-like effector (TALE) regulation
por: Lonzarić, Jan, et al.
Publicado: (2016)

Generation of knockout rabbits using transcription activator-like effector nucleases
por: Wang, Yu, et al.
Publicado: (2014)

A transcription activator-like effector induction system mediated by proteolysis
por: Copeland, Matthew F., et al.
Publicado: (2016)

Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine
por: Okitsu, Cindy Y, et al.
Publicado: (2015)

Direct Sensing of 5-Methylcytosine by Polymerase Chain Reaction**
por: Aschenbrenner, Joos, et al.
Publicado: (2014)

Variable presence of 5-methylcytosine in commercial RNA and DNA
por: Schmid, Katharina, et al.
Publicado: (2015)

Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA
por: Matsuda, Shigeru, et al.
Publicado: (2018)

Formation and determination of the oxidation products of 5-methylcytosine in RNA
por: Huang, Wei, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_ejli1f9piol042tf6pta7fvga5