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In Vivo Genome Editing in Type I and II Methanotrophs Using a CRISPR/Cas9 System

[Image: see text] Methanotrophic bacteria are Gram-negative, aerobic organisms that use methane as their sole source of carbon and energy. In this study, we constructed and exemplified a CRISPR/Cas9 genome editing system and used it to successfully make gene deletions and insertions in the type I me...

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Detalles Bibliográficos
Autores principales: Rumah, Bashir L., Claxton Stevens, Benedict H., Yeboah, Jake E., Stead, Christopher E., Harding, Emily L., Minton, Nigel P., Zhang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942187/
https://www.ncbi.nlm.nih.gov/pubmed/36688528
http://dx.doi.org/10.1021/acssynbio.2c00554