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Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator

[Image: see text] Yeast surface display is a valuable tool for protein engineering and directed evolution; however, significant variability in the copy number (i.e., avidity) of displayed variants on the yeast cell wall complicates screening and selection campaigns. Here, we report an engineered tit...

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Detalles Bibliográficos
Autores principales: Lopez-Morales, Joanan, Vanella, Rosario, Kovacevic, Gordana, Santos, Mariana Sá, Nash, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942200/
https://www.ncbi.nlm.nih.gov/pubmed/36728831
http://dx.doi.org/10.1021/acssynbio.2c00351