Cargando…

The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune Cells

[Image: see text] CRISPR-Cas12a nucleases have expanded the toolbox for targeted genome engineering in a broad range of organisms. Here, using a high-throughput engineering approach, we explored the potential of a novel CRISPR-MAD7 system for genome editing in human cells. We evaluated several thous...

Descripción completa

Detalles Bibliográficos
Autores principales:	Mohr, Marina, Damas, Nkerorema, Gudmand-Høyer, Johanne, Zeeberg, Katrine, Jedrzejczyk, Dominika, Vlassis, Arsenios, Morera-Gómez, Martí, Pereira-Schoning, Sara, Puš, Urška, Oliver-Almirall, Anna, Lyholm Jensen, Tanja, Baumgartner, Roland, Tate Weinert, Brian, Gill, Ryan T., Warnecke, Tanya
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942205/ https://www.ncbi.nlm.nih.gov/pubmed/36750230 http://dx.doi.org/10.1021/acssynbio.2c00179

Ejemplares similares

CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cells
por: Vlassis, Arsenios, et al.
Publicado: (2023)

CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA
por: Jedrzejczyk, D. J., et al.
Publicado: (2022)

Vitamin D Inhibits IL-22 Production Through a Repressive Vitamin D Response Element in the il22 Promoter
por: Lopez, Daniel V., et al.
Publicado: (2021)

Functional Interplay between RNA Viruses and Non-Coding RNA in Mammals
por: Damas, Nkerorema Djodji, et al.
Publicado: (2019)

The general control nonderepressible-2 kinase mediates stress response and longevity induced by target of rapamycin inactivation in Caenorhabditis elegans
por: Rousakis, Aris, et al.
Publicado: (2013)

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery
por: Graczyk, Anna, et al.
Publicado: (2020)

Case Report: First longitudinal study of a patient with CALR positive clonal hematopoiesis of indeterminate potential developing into pre-fibrotic myelofibrosis
por: Kjær, Lasse, et al.
Publicado: (2023)

A Concise Introduction to Multiagent Systems and Distributed Artificial Intelligence
por: Vlassis, Nikos
Publicado: (2007)

Advanced Photocatalytic Materials
por: Likodimos, Vlassis
Publicado: (2020)

Organic acid toxicity, tolerance, and production in Escherichia coli biorefining applications
por: Warnecke, Tanya, et al.
Publicado: (2005)

SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization
por: Damas, Nkerorema Djodji, et al.
Publicado: (2016)

Validation of the Slovenian Version of the Movement Imagery Questionnaire for Children (MIQ-C): A Measurement Tool to Assess the Imagery Ability of Motor Tasks in Children
por: Šlosar, Luka, et al.
Publicado: (2023)

Transferrin-Bound Doxorubicin Enhances Apoptosis and DNA Damage through the Generation of Pro-Inflammatory Responses in Human Leukemia Cells
por: Jedrzejczyk, Monika, et al.
Publicado: (2020)

Intranasal insulin neuroprotection in ischemic stroke
por: Lioutas, Vasileios-Arsenios, et al.
Publicado: (2016)

The use of tensiomyography in older adults: a systematic review
por: Pus, Katarina, et al.
Publicado: (2023)

TGFβ Signaling Increases Net Acid Extrusion, Proliferation and Invasion in Panc-1 Pancreatic Cancer Cells: SMAD4 Dependence and Link to Merlin/NF2 Signaling
por: Malinda, Raj R., et al.
Publicado: (2020)

Mathematical modelling as a proof of concept for MPNs as a human inflammation model for cancer development
por: Andersen, Morten, et al.
Publicado: (2017)

Promoting interactive skills and mind-mindedness among early childcare professionals: study protocol for a randomized wait-list controlled trial comparing the Circle of Security approach with care as usual in center-based childcare (the SECURE project)
por: Smith-Nielsen, Johanne, et al.
Publicado: (2022)

Dynamics of disasters: impact, risk, resilience, and solutions
por: Kotsireas, Ilias, et al.
Publicado: (2021)

Data‐driven analysis of JAK2V617F kinetics during interferon‐alpha2 treatment of patients with polycythemia vera and related neoplasms
por: Pedersen, Rasmus K., et al.
Publicado: (2020)

fastGapFill: efficient gap filling in metabolic networks
por: Thiele, Ines, et al.
Publicado: (2014)

Fast Reconstruction of Compact Context-Specific Metabolic Network Models
por: Vlassis, Nikos, et al.
Publicado: (2014)

Accessibility and interventions of crisis resolution teams: a multicenter study of team practices and team differences in Norway
por: Ruud, Torleif, et al.
Publicado: (2022)

Characteristics and Diabetes Control in Adults With Type 1 Diabetes Admitted With COVID-19 Infection
por: Vamvini, Maria, et al.
Publicado: (2020)

Tobacco advertising, promotion, and sponsorship ban adoption: A pilot study of the reporting challenges faced by low- and middle-income countries
por: Tselengidis, Arsenios, et al.
Publicado: (2023)

Cancer Associated Fibroblast (CAF) Regulation of PDAC Parenchymal (CPC) and CSC Phenotypes Is Modulated by ECM Composition
por: Cannone, Stefania, et al.
Publicado: (2022)

Mineral and Bone Disease in Black African Hemodialysis Patients
por: Almirall, Jaume
Publicado: (2013)

A Bioinformatics-Based Alternative mRNA Splicing Code that May Explain Some Disease Mutations Is Conserved in Animals
por: Qu, Wen, et al.
Publicado: (2017)

Alignment-free Visualization of Metagenomic Data by Nonlinear Dimension Reduction
por: Laczny, Cedric C., et al.
Publicado: (2014)

Editorial: Understanding PFO-associated stroke
por: Karapanayiotides, Theodoros, et al.
Publicado: (2023)

Comparison of the Tribological Properties of the Thermal Diffusion Zinc Coating to the Classic and Heat Treated Hot-Dip Zinc Coatings
por: Jędrzejczyk, Dariusz, et al.
Publicado: (2021)

The Comparative Analysis of the Coatings Deposited on the Automotive Parts by the Cataphoresis Method
por: Skotnicki, Wojciech, et al.
Publicado: (2021)

The Impact of Heat Treatment on the Behavior of a Hot-Dip Zinc Coating Applied to Steel During Dry Friction
por: Jędrzejczyk, Dariusz, et al.
Publicado: (2021)

The Influence of Heat Treatment on Corrosion Resistance and Microhardness of Hot-Dip Zinc Coating Deposited on Steel Bolts
por: Jędrzejczyk, Dariusz, et al.
Publicado: (2022)

Clinician compliance to recommendations regarding the risk of suicidality with selective serotonin reuptake inhibitors in the treatment of children and adolescents
por: Sørensen, Johanne Østerby, et al.
Publicado: (2019)

Gene Expression Correlations in Human Cancer Cell Lines Define Molecular Interaction Networks for Epithelial Phenotype
por: Kohn, Kurt W., et al.
Publicado: (2014)

Barriers to resilience among healthcare staff in Ireland in the post-austerity period
por: Almirall Sanchez, A
Publicado: (2023)

An apprenticeship model in the training of psychotherapy students. Study protocol for a randomized controlled trial and qualitative investigation
por: Brattland, Heidi, et al.
Publicado: (2022)

A Bayesian Geostatistical Moran Curve Model for Estimating Net Changes of Tsetse Populations in Zambia
por: Sedda, Luigi, et al.
Publicado: (2014)

Effects of Intensive and Semi-Intensive Production on Sheep Milk Chemical Composition, Physicochemical Characteristics, Fatty Acid Profile, and Nutritional Indices
por: Kasapidou, Eleni, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_958ek7qpf0sfo82ing3o96cl6k