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Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics
Fluorescence correlation spectroscopy is a versatile tool for studying fast conformational changes of biomolecules especially when combined with Förster resonance energy transfer (FRET). Despite the many methods available for identifying structural dynamics in FRET experiments, the determination of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942831/ https://www.ncbi.nlm.nih.gov/pubmed/36652471 http://dx.doi.org/10.1073/pnas.2211896120 |