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Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics

Fluorescence correlation spectroscopy is a versatile tool for studying fast conformational changes of biomolecules especially when combined with Förster resonance energy transfer (FRET). Despite the many methods available for identifying structural dynamics in FRET experiments, the determination of...

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Detalles Bibliográficos
Autores principales: Schröder, Tim, Bohlen, Johann, Ochmann, Sarah E., Schüler, Patrick, Krause, Stefan, Lamb, Don C., Tinnefeld, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942831/
https://www.ncbi.nlm.nih.gov/pubmed/36652471
http://dx.doi.org/10.1073/pnas.2211896120