CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice

In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles a...

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Detalles Bibliográficos
Autores principales: Cassidy, Annelise, Pelletier, Stephane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943854/
https://www.ncbi.nlm.nih.gov/pubmed/36853660
http://dx.doi.org/10.1016/j.xpro.2023.102116

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943854/
https://www.ncbi.nlm.nih.gov/pubmed/36853660
http://dx.doi.org/10.1016/j.xpro.2023.102116

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