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CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice
In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles a...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943854/ https://www.ncbi.nlm.nih.gov/pubmed/36853660 http://dx.doi.org/10.1016/j.xpro.2023.102116 |