Optimized lentiviral vector transduction of adherent cells and analysis in sulforhodamine B proliferation and chromatin immunoprecipitation assays

Transduction with lentiviral vectors is a useful approach to study the molecular function of specific genes in mammalian cells. Here, we present a calcium phosphate-based transfection protocol that guarantees highly efficient production and delivery of lentiviral vectors in adherent cultured cells....

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Luyuan, Trent, Jonathan C., Eid, Josiane E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943855/
https://www.ncbi.nlm.nih.gov/pubmed/36853730
http://dx.doi.org/10.1016/j.xpro.2023.102109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943855/
https://www.ncbi.nlm.nih.gov/pubmed/36853730
http://dx.doi.org/10.1016/j.xpro.2023.102109

Ejemplares similares