Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors

Viral vectors hold enormous potential for genome editing in plants as transient delivery vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral vectors for single-guide RNA (sgRNA) delivery. The obtained viral constructs are based on compact T-DNA binary vectors of t...

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Detalles Bibliográficos
Autores principales: Uranga, Mireia, Aragonés, Verónica, Daròs, José- Antonio, Pasin, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943877/
https://www.ncbi.nlm.nih.gov/pubmed/36853698
http://dx.doi.org/10.1016/j.xpro.2023.102091

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943877/
https://www.ncbi.nlm.nih.gov/pubmed/36853698
http://dx.doi.org/10.1016/j.xpro.2023.102091

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