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Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors

Viral vectors hold enormous potential for genome editing in plants as transient delivery vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral vectors for single-guide RNA (sgRNA) delivery. The obtained viral constructs are based on compact T-DNA binary vectors of t...

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Detalles Bibliográficos
Autores principales:	Uranga, Mireia, Aragonés, Verónica, Daròs, José- Antonio, Pasin, Fabio
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943877/ https://www.ncbi.nlm.nih.gov/pubmed/36853698 http://dx.doi.org/10.1016/j.xpro.2023.102091

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