A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva

In response to the SARS-CoV-2 pandemic, we developed a multiplexed, paired-pool droplet digital PCR (MP4) screening assay. Key features of our assay are the use of minimally processed saliva, 8-sample paired pools, and reverse-transcription droplet digital PCR (RT-ddPCR) targeting the SARS-CoV-2 nuc...

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Detalles Bibliográficos
Autores principales: Wagner, Kaitlyn, Fox, Phil, Gordon, Elizabeth, Hahn, Westen, Olsen, Kenzie, Markham, Alex, Buglewicz, Dylan, Selemenakis, Platon, Lessard, Avery, Goldstein, Daniella, Threatt, Alissa, Davis, Luke, Miller-Dawson, Jake, Stockett, Halie, Rugh, Kristin, Turner, Houston, Remias, Michelle, Williams, Maggie, Chavez, Jorge, Galindo, Gabriel, Cialek, Charlotte, Koch, Amanda, Fout, Alex, Fosdick, Bailey, Broeckling, Bettina, Zabel, Mark D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944410/
https://www.ncbi.nlm.nih.gov/pubmed/36813822
http://dx.doi.org/10.1038/s41598-023-29858-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944410/
https://www.ncbi.nlm.nih.gov/pubmed/36813822
http://dx.doi.org/10.1038/s41598-023-29858-5

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