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Heterologous Expression of Recombinant Human Cytochrome P450 (CYP) in Escherichia coli: N-Terminal Modification, Expression, Isolation, Purification, and Reconstitution

Cytochrome P450 (CYP) enzymes play important roles in metabolising endogenous and xenobiotic substances. Characterisations of human CYP proteins have been advanced with the rapid development of molecular technology that allows heterologous expression of human CYPs. Among several hosts, bacteria syst...

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Detalles Bibliográficos
Autores principales: Shang, Tao, Fang, Chee Mun, Ong, Chin Eng, Pan, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944785/
https://www.ncbi.nlm.nih.gov/pubmed/36810444
http://dx.doi.org/10.3390/biotech12010017