An integrated workflow for phosphopeptide identification in natural killer cells (NK-92MI) and their targets (MDA-MB-231) during immunological synapse formation

Here, we present a protocol to identify and quantify phosphopeptides during the dynamic formation of an immunological synapse. We describe steps for mixing isotope-labeled immune and target cells, the stabilization of cell-to-cell conjugates by cross-linking, and their isolation by fluorescence-acti...

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Detalles Bibliográficos
Autores principales: Perez-Hernandez, Daniel, Filali, Liza, Thomas, Clement, Dittmar, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944981/
https://www.ncbi.nlm.nih.gov/pubmed/36853697
http://dx.doi.org/10.1016/j.xpro.2023.102104

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944981/
https://www.ncbi.nlm.nih.gov/pubmed/36853697
http://dx.doi.org/10.1016/j.xpro.2023.102104

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