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Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology

Clonality assessment using the unique rearrangements of immunoglobulin (IG) and T-cell receptor (TR) genes in lymphocytes is a widely applied supplementary test for the diagnosis of B-cell and T-cell lymphoma. To enable a more sensitive detection and a more precise comparison of clones compared with...

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Autores principales: Groenen, Patricia J. T. A., van den Brand, Michiel, Kroeze, Leonie I., Amir, Avital L., Hebeda, Konnie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945094/
https://www.ncbi.nlm.nih.gov/pubmed/36845702
http://dx.doi.org/10.3389/fonc.2023.1107171
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author Groenen, Patricia J. T. A.
van den Brand, Michiel
Kroeze, Leonie I.
Amir, Avital L.
Hebeda, Konnie M.
author_facet Groenen, Patricia J. T. A.
van den Brand, Michiel
Kroeze, Leonie I.
Amir, Avital L.
Hebeda, Konnie M.
author_sort Groenen, Patricia J. T. A.
collection PubMed
description Clonality assessment using the unique rearrangements of immunoglobulin (IG) and T-cell receptor (TR) genes in lymphocytes is a widely applied supplementary test for the diagnosis of B-cell and T-cell lymphoma. To enable a more sensitive detection and a more precise comparison of clones compared with conventional clonality analysis based on fragment analysis, the EuroClonality NGS Working Group developed and validated a next-generation sequencing (NGS)-based clonality assay for detection of the IG heavy and kappa light chain and TR gene rearrangements for formalin-fixed and paraffin-embedded tissues. We outline the features and advantages of NGS-based clonality detection and discuss potential applications for NGS-based clonality testing in pathology, including site specific lymphoproliferations, immunodeficiency and autoimmune disease and primary and relapsed lymphomas. Also, we briefly discuss the role of T-cell repertoire of reactive lymphocytic infiltrations in solid tumors and B-lymphoma.
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spelling pubmed-99450942023-02-23 Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology Groenen, Patricia J. T. A. van den Brand, Michiel Kroeze, Leonie I. Amir, Avital L. Hebeda, Konnie M. Front Oncol Oncology Clonality assessment using the unique rearrangements of immunoglobulin (IG) and T-cell receptor (TR) genes in lymphocytes is a widely applied supplementary test for the diagnosis of B-cell and T-cell lymphoma. To enable a more sensitive detection and a more precise comparison of clones compared with conventional clonality analysis based on fragment analysis, the EuroClonality NGS Working Group developed and validated a next-generation sequencing (NGS)-based clonality assay for detection of the IG heavy and kappa light chain and TR gene rearrangements for formalin-fixed and paraffin-embedded tissues. We outline the features and advantages of NGS-based clonality detection and discuss potential applications for NGS-based clonality testing in pathology, including site specific lymphoproliferations, immunodeficiency and autoimmune disease and primary and relapsed lymphomas. Also, we briefly discuss the role of T-cell repertoire of reactive lymphocytic infiltrations in solid tumors and B-lymphoma. Frontiers Media S.A. 2023-02-08 /pmc/articles/PMC9945094/ /pubmed/36845702 http://dx.doi.org/10.3389/fonc.2023.1107171 Text en Copyright © 2023 Groenen, van den Brand, Kroeze, Amir and Hebeda https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Groenen, Patricia J. T. A.
van den Brand, Michiel
Kroeze, Leonie I.
Amir, Avital L.
Hebeda, Konnie M.
Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
title Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
title_full Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
title_fullStr Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
title_full_unstemmed Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
title_short Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
title_sort read the clonotype: next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945094/
https://www.ncbi.nlm.nih.gov/pubmed/36845702
http://dx.doi.org/10.3389/fonc.2023.1107171
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