Cargando…

Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation Sequencing

[Image: see text] Recently, we constructed a hybrid thymine DNA glycosylase (hyTDG) by linking a 29-amino acid sequence from the human thymine DNA glycosylase with the catalytic domain of DNA mismatch glycosylase (MIG) from M. thermoautotrophicum, increasing the overall activity of the glycosylase....

Descripción completa

Detalles Bibliográficos
Autores principales:	Baljinnyam, Tuvshintugs, Conrad, James W., Sowers, Mark L., Chang-Gu, Bruce, Herring, Jason L., Hackfeld, Linda C., Zhang, Kangling, Sowers, Lawrence C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945173/ https://www.ncbi.nlm.nih.gov/pubmed/36647573 http://dx.doi.org/10.1021/acs.chemrestox.2c00172

Ejemplares similares

Measurement of deaminated cytosine adducts in DNA using a novel hybrid thymine DNA glycosylase
por: Hsu, Chia Wei, et al.
Publicado: (2022)

Chemical and enzymatic modifications of 5-methylcytosine at the intersection of DNA damage, repair, and epigenetic reprogramming
por: Baljinnyam, Tuvshintugs, et al.
Publicado: (2022)

A combinatorial system to examine the enzymatic repair of multiply damaged DNA substrates
por: Hsu, Chia Wei, et al.
Publicado: (2022)

DNA Base Excision Repair Intermediates Influence Duplex–Quadruplex Equilibrium
por: Sowers, Mark L., et al.
Publicado: (2023)

Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection
por: Tang, Hui, et al.
Publicado: (2016)

Glioblastoma and Methionine Addiction
por: Sowers, Mark L., et al.
Publicado: (2022)

Traumatic brain injury induces region-specific glutamate metabolism changes as measured by multiple mass spectrometry methods
por: Sowers, James L., et al.
Publicado: (2021)

The effect of Pot1 binding on the repair of thymine analogs in a telomeric DNA sequence
por: Theruvathu, Jacob A., et al.
Publicado: (2014)

Proteomic changes in traumatic brain injury: experimental approaches
por: Sowers, James L., et al.
Publicado: (2018)

Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans
por: Raj, Hans, et al.
Publicado: (2011)

Transition Mutations in the hTERT Promoter Are Unrelated to Potential i-motif Formation in the C-Rich Strand
por: Conrad, James W., et al.
Publicado: (2023)

Expression and Characterization of an Alginate Lyase and Its Thermostable Mutant in Pichia pastoris
por: Yang, Suxiao, et al.
Publicado: (2020)

Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming
por: Xu, Huan, et al.
Publicado: (2022)

A Microfluidic DNA Library Preparation Platform for Next-Generation Sequencing
por: Kim, Hanyoup, et al.
Publicado: (2013)

Norepinephrine Reduces Reactive Oxygen Species (ROS) and DNA Damage in Ovarian Surface Epithelial Cells
por: Patel, Pooja R, et al.
Publicado: (2015)

Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing
por: Mohr, Sabine, et al.
Publicado: (2013)

Sex-Specific Proteomic Changes Induced by Genetic Deletion of Fibroblast Growth Factor 14 (FGF14), a Regulator of Neuronal Ion Channels
por: Sowers, Mark L., et al.
Publicado: (2019)

Enzymatic depolymerization of alginate by two novel thermostable alginate lyases from Rhodothermus marinus
por: Dobruchowska, Justyna M., et al.
Publicado: (2022)

Improving the thermostability of alginate lyase FlAlyA with high expression by computer-aided rational design for industrial preparation of alginate oligosaccharides
por: Zhang, Xiu, et al.
Publicado: (2022)

Thermostable DNA ligases from hyperthermophiles in biotechnology
por: Shi, Jingru, et al.
Publicado: (2023)

Multi-OMICs analysis reveals metabolic and epigenetic changes associated with macrophage polarization
por: Sowers, Mark L., et al.
Publicado: (2022)

Modeling‐Assisted Design of Thermostable Benzaldehyde Lyases from Rhodococcus erythropolis for Continuous Production of α‐Hydroxy Ketones
por: Peng, Martin, et al.
Publicado: (2021)

Next generation DNA led technologies
por: Avadhanam, Sharada, et al.
Publicado: (2016)

Sirtuin-dependent metabolic and epigenetic regulation of macrophages during tuberculosis
por: Zhang, Kangling, et al.
Publicado: (2023)

Short-time geometry of random heat kernels
por: Sowers, Richard B, et al.
Publicado: (1998)

Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA
por: Star, Bastiaan, et al.
Publicado: (2014)

Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
por: Miura, Fumihito, et al.
Publicado: (2018)

Preparing the Next Generation of Neuroscience Educators
por: Hardwick, Jean C.
Publicado: (2005)

Non-random DNA fragmentation in next-generation sequencing
por: Poptsova, Maria S., et al.
Publicado: (2014)

Special Issue: Next Generation DNA Sequencing
por: Richardson, Paul
Publicado: (2010)

Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
por: Shokralla, Shadi, et al.
Publicado: (2014)

Correction: Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA
Publicado: (2014)

Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability
por: Zhou, Zhanping, et al.
Publicado: (2021)

Environmental Politics in Egypt
por: Sowers, Jeannie
Publicado: (2013)

PCR performance of a thermostable heterodimeric archaeal DNA polymerase
por: Killelea, Tom, et al.
Publicado: (2014)

Preparing Unbiased T-Cell Receptor and Antibody cDNA Libraries for the Deep Next Generation Sequencing Profiling
por: Mamedov, Ilgar Z., et al.
Publicado: (2013)

Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation
por: Suckling, Lorna, et al.
Publicado: (2019)

DNA polymerase β uses its lyase domain in a processive search for DNA damage
por: Howard, Michael J., et al.
Publicado: (2017)

Preparing for the next generation pharmacists
por: DiPiro, Joseph T.
Publicado: (2020)

Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods
por: McDonough, Samantha J., et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_kkn60iekkuvuidh1unqpug9hvi