Activity-based CRISPR scanning uncovers allostery in DNA methylation maintenance machinery

Allostery enables dynamic control of protein function. A paradigmatic example is the tightly orchestrated process of DNA methylation maintenance. Despite the fundamental importance of allosteric sites, their identification remains highly challenging. Here, we perform CRISPR scanning on the essential...

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Detalles Bibliográficos
Autores principales: Ngan, Kevin Chun-Ho, Hoenig, Samuel M, Kwok, Hui Si, Lue, Nicholas Z, Gosavi, Pallavi M, Tanner, David A, Garcia, Emma M, Lee, Ceejay, Liau, Brian B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946446/
https://www.ncbi.nlm.nih.gov/pubmed/36762644
http://dx.doi.org/10.7554/eLife.80640

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946446/
https://www.ncbi.nlm.nih.gov/pubmed/36762644
http://dx.doi.org/10.7554/eLife.80640

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