Targeting DNA repair pathways with B02 and Nocodazole small molecules to improve CRIS-PITCh mediated cassette integration in CHO-K1 cells

CRISPR-mediated integration could be used to develop the recombinant CHO (rCHO) cells by knock-in into the hotspot loci. However, low HDR efficiency besides the complex donor design is the main barrier for achieving so. The recently introduced MMEJ-mediated CRISPR system (CRIS-PITCh) uses a donor wi...

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Detalles Bibliográficos
Autores principales: Rahmani, Behnaz, Kheirandish, Mohammad Hassan, Ghanbari, Samaneh, Raz, Abbasali, Shamsi, Fahimeh, Davami, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947112/
https://www.ncbi.nlm.nih.gov/pubmed/36813818
http://dx.doi.org/10.1038/s41598-023-29863-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947112/
https://www.ncbi.nlm.nih.gov/pubmed/36813818
http://dx.doi.org/10.1038/s41598-023-29863-8

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