Cell-free reconstitution of peroxisomal matrix protein import using Xenopus egg extract

Peroxisomes are vital metabolic organelles whose matrix enzymes are imported from the cytosol in a folded state by the soluble receptor PEX5. The import mechanism has been challenging to decipher because of the lack of suitable in vitro systems. Here, we present a protocol for reconstituting matrix...

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Detalles Bibliográficos
Autores principales: Skowyra, Michael L., Rapoport, Tom A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947420/
https://www.ncbi.nlm.nih.gov/pubmed/36853666
http://dx.doi.org/10.1016/j.xpro.2023.102111

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947420/
https://www.ncbi.nlm.nih.gov/pubmed/36853666
http://dx.doi.org/10.1016/j.xpro.2023.102111

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