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Cell-free reconstitution of peroxisomal matrix protein import using Xenopus egg extract
Peroxisomes are vital metabolic organelles whose matrix enzymes are imported from the cytosol in a folded state by the soluble receptor PEX5. The import mechanism has been challenging to decipher because of the lack of suitable in vitro systems. Here, we present a protocol for reconstituting matrix...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947420/ https://www.ncbi.nlm.nih.gov/pubmed/36853666 http://dx.doi.org/10.1016/j.xpro.2023.102111 |