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OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY
We identify thermal magnetic field fluctuations, caused by thermal electron motion (“Johnson noise”) in electrically conductive materials, as a potential resolution limit in transmission electron microscopy with a phase plate. Specifically, resolution loss can occur if the electron diffraction patte...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9949102/ https://www.ncbi.nlm.nih.gov/pubmed/36824829 http://dx.doi.org/10.1101/2023.02.12.528160 |
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author | Axelrod, Jeremy J. Petrov, Petar N. Zhang, Jessie T. Remis, Jonathan Buijsse, Bart Glaeser, Robert M. Mȕller, Holger |
author_facet | Axelrod, Jeremy J. Petrov, Petar N. Zhang, Jessie T. Remis, Jonathan Buijsse, Bart Glaeser, Robert M. Mȕller, Holger |
author_sort | Axelrod, Jeremy J. |
collection | PubMed |
description | We identify thermal magnetic field fluctuations, caused by thermal electron motion (“Johnson noise”) in electrically conductive materials, as a potential resolution limit in transmission electron microscopy with a phase plate. Specifically, resolution loss can occur if the electron diffraction pattern is magnified to extend phase contrast to lower spatial frequencies, and if conductive materials are placed too close to the electron beam. While our initial implementation of a laser phase plate (LPP) was significantly affected by these factors, a redesign eliminated the problem and brought the performance close to the expected level. The resolution now appears to be limited by residual Johnson noise arising from the electron beam liner tube in the region of the LPP, together with the chromatic aberration of the relay optics. These two factors can be addressed during future development of the LPP. |
format | Online Article Text |
id | pubmed-9949102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-99491022023-02-24 OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY Axelrod, Jeremy J. Petrov, Petar N. Zhang, Jessie T. Remis, Jonathan Buijsse, Bart Glaeser, Robert M. Mȕller, Holger bioRxiv Article We identify thermal magnetic field fluctuations, caused by thermal electron motion (“Johnson noise”) in electrically conductive materials, as a potential resolution limit in transmission electron microscopy with a phase plate. Specifically, resolution loss can occur if the electron diffraction pattern is magnified to extend phase contrast to lower spatial frequencies, and if conductive materials are placed too close to the electron beam. While our initial implementation of a laser phase plate (LPP) was significantly affected by these factors, a redesign eliminated the problem and brought the performance close to the expected level. The resolution now appears to be limited by residual Johnson noise arising from the electron beam liner tube in the region of the LPP, together with the chromatic aberration of the relay optics. These two factors can be addressed during future development of the LPP. Cold Spring Harbor Laboratory 2023-02-13 /pmc/articles/PMC9949102/ /pubmed/36824829 http://dx.doi.org/10.1101/2023.02.12.528160 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Axelrod, Jeremy J. Petrov, Petar N. Zhang, Jessie T. Remis, Jonathan Buijsse, Bart Glaeser, Robert M. Mȕller, Holger OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY |
title | OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY |
title_full | OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY |
title_fullStr | OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY |
title_full_unstemmed | OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY |
title_short | OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY |
title_sort | overcoming resolution loss due to thermal magnetic field fluctuations from phase plates in transmission electron microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9949102/ https://www.ncbi.nlm.nih.gov/pubmed/36824829 http://dx.doi.org/10.1101/2023.02.12.528160 |
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