Cargando…

OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY

We identify thermal magnetic field fluctuations, caused by thermal electron motion (“Johnson noise”) in electrically conductive materials, as a potential resolution limit in transmission electron microscopy with a phase plate. Specifically, resolution loss can occur if the electron diffraction patte...

Descripción completa

Detalles Bibliográficos
Autores principales: Axelrod, Jeremy J., Petrov, Petar N., Zhang, Jessie T., Remis, Jonathan, Buijsse, Bart, Glaeser, Robert M., Mȕller, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9949102/
https://www.ncbi.nlm.nih.gov/pubmed/36824829
http://dx.doi.org/10.1101/2023.02.12.528160
_version_ 1784892906928078848
author Axelrod, Jeremy J.
Petrov, Petar N.
Zhang, Jessie T.
Remis, Jonathan
Buijsse, Bart
Glaeser, Robert M.
Mȕller, Holger
author_facet Axelrod, Jeremy J.
Petrov, Petar N.
Zhang, Jessie T.
Remis, Jonathan
Buijsse, Bart
Glaeser, Robert M.
Mȕller, Holger
author_sort Axelrod, Jeremy J.
collection PubMed
description We identify thermal magnetic field fluctuations, caused by thermal electron motion (“Johnson noise”) in electrically conductive materials, as a potential resolution limit in transmission electron microscopy with a phase plate. Specifically, resolution loss can occur if the electron diffraction pattern is magnified to extend phase contrast to lower spatial frequencies, and if conductive materials are placed too close to the electron beam. While our initial implementation of a laser phase plate (LPP) was significantly affected by these factors, a redesign eliminated the problem and brought the performance close to the expected level. The resolution now appears to be limited by residual Johnson noise arising from the electron beam liner tube in the region of the LPP, together with the chromatic aberration of the relay optics. These two factors can be addressed during future development of the LPP.
format Online
Article
Text
id pubmed-9949102
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-99491022023-02-24 OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY Axelrod, Jeremy J. Petrov, Petar N. Zhang, Jessie T. Remis, Jonathan Buijsse, Bart Glaeser, Robert M. Mȕller, Holger bioRxiv Article We identify thermal magnetic field fluctuations, caused by thermal electron motion (“Johnson noise”) in electrically conductive materials, as a potential resolution limit in transmission electron microscopy with a phase plate. Specifically, resolution loss can occur if the electron diffraction pattern is magnified to extend phase contrast to lower spatial frequencies, and if conductive materials are placed too close to the electron beam. While our initial implementation of a laser phase plate (LPP) was significantly affected by these factors, a redesign eliminated the problem and brought the performance close to the expected level. The resolution now appears to be limited by residual Johnson noise arising from the electron beam liner tube in the region of the LPP, together with the chromatic aberration of the relay optics. These two factors can be addressed during future development of the LPP. Cold Spring Harbor Laboratory 2023-02-13 /pmc/articles/PMC9949102/ /pubmed/36824829 http://dx.doi.org/10.1101/2023.02.12.528160 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Axelrod, Jeremy J.
Petrov, Petar N.
Zhang, Jessie T.
Remis, Jonathan
Buijsse, Bart
Glaeser, Robert M.
Mȕller, Holger
OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY
title OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY
title_full OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY
title_fullStr OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY
title_full_unstemmed OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY
title_short OVERCOMING RESOLUTION LOSS DUE TO THERMAL MAGNETIC FIELD FLUCTUATIONS FROM PHASE PLATES IN TRANSMISSION ELECTRON MICROSCOPY
title_sort overcoming resolution loss due to thermal magnetic field fluctuations from phase plates in transmission electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9949102/
https://www.ncbi.nlm.nih.gov/pubmed/36824829
http://dx.doi.org/10.1101/2023.02.12.528160
work_keys_str_mv AT axelrodjeremyj overcomingresolutionlossduetothermalmagneticfieldfluctuationsfromphaseplatesintransmissionelectronmicroscopy
AT petrovpetarn overcomingresolutionlossduetothermalmagneticfieldfluctuationsfromphaseplatesintransmissionelectronmicroscopy
AT zhangjessiet overcomingresolutionlossduetothermalmagneticfieldfluctuationsfromphaseplatesintransmissionelectronmicroscopy
AT remisjonathan overcomingresolutionlossduetothermalmagneticfieldfluctuationsfromphaseplatesintransmissionelectronmicroscopy
AT buijssebart overcomingresolutionlossduetothermalmagneticfieldfluctuationsfromphaseplatesintransmissionelectronmicroscopy
AT glaeserrobertm overcomingresolutionlossduetothermalmagneticfieldfluctuationsfromphaseplatesintransmissionelectronmicroscopy
AT mullerholger overcomingresolutionlossduetothermalmagneticfieldfluctuationsfromphaseplatesintransmissionelectronmicroscopy