Programmable RNA detection with CRISPR-Cas12a

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CRISPR is a prominent bioengineering tool and the type V CRISPR-associated protein complex, Cas12a, is widely used in diagnostic platforms due to its innate ability to cleave DNA substrates. Here we demonstrate that Cas12a can also be programmed to directly detect RNA substrates without the need for...

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Detalles Bibliográficos
Autores principales: Rananaware, Santosh R., Vesco, Emma K., Shoemaker, Grace M., Anekar, Swapnil S., Sandoval, Luke Samuel W., Meister, Katelyn S., Macaluso, Nicolas C., Nguyen, Long T., Jain, Piyush K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9949221/
https://www.ncbi.nlm.nih.gov/pubmed/36824842
http://dx.doi.org/10.21203/rs.3.rs-2549171/v1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9949221/
https://www.ncbi.nlm.nih.gov/pubmed/36824842
http://dx.doi.org/10.21203/rs.3.rs-2549171/v1

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