A High-Throughput Assay for In Vitro Determination of Release Factor-Dependent Peptide Release from a Pretermination Complex by Fluorescence Anisotropy—Application to Nonsense Suppressor Screening and Mechanistic Studies

Premature termination codons (PTCs) account for ~12% of all human disease mutations. Translation readthrough-inducing drugs (TRIDs) are prominent among the several therapeutic approaches being used to overcome PTCs. Ataluren is the only TRID that has been approved for treating patients suffering fro...

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Detalles Bibliográficos
Autores principales: Ghelfi, Mikel D., Bhat, Saleem Y., Li, Hong, Cooperman, Barry S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953321/
https://www.ncbi.nlm.nih.gov/pubmed/36830611
http://dx.doi.org/10.3390/biom13020242

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953321/
https://www.ncbi.nlm.nih.gov/pubmed/36830611
http://dx.doi.org/10.3390/biom13020242

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