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Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli

Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and im...

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Detalles Bibliográficos
Autores principales: Bruggeman, Christine W., Haasnoot, Guus H., Peterman, Erwin J.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958070/
https://www.ncbi.nlm.nih.gov/pubmed/36853676
http://dx.doi.org/10.1016/j.xpro.2023.102121