Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli

Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and im...

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Detalles Bibliográficos
Autores principales: Bruggeman, Christine W., Haasnoot, Guus H., Peterman, Erwin J.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958070/
https://www.ncbi.nlm.nih.gov/pubmed/36853676
http://dx.doi.org/10.1016/j.xpro.2023.102121
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author Bruggeman, Christine W.
Haasnoot, Guus H.
Peterman, Erwin J.G.
author_facet Bruggeman, Christine W.
Haasnoot, Guus H.
Peterman, Erwin J.G.
author_sort Bruggeman, Christine W.
collection PubMed
description Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and imaging of C. elegans through fluorescence microscopy to determine their molecular and/or cellular response to chemosensory stimuli. This protocol can also be applied to amphid neurons by inserting the worm in the chip head-first. For complete details on the use and execution of this protocol, please refer to Bruggeman et al. (2022).(1)
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spelling pubmed-99580702023-02-26 Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli Bruggeman, Christine W. Haasnoot, Guus H. Peterman, Erwin J.G. STAR Protoc Protocol Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and imaging of C. elegans through fluorescence microscopy to determine their molecular and/or cellular response to chemosensory stimuli. This protocol can also be applied to amphid neurons by inserting the worm in the chip head-first. For complete details on the use and execution of this protocol, please refer to Bruggeman et al. (2022).(1) Elsevier 2023-02-14 /pmc/articles/PMC9958070/ /pubmed/36853676 http://dx.doi.org/10.1016/j.xpro.2023.102121 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Bruggeman, Christine W.
Haasnoot, Guus H.
Peterman, Erwin J.G.
Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
title Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
title_full Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
title_fullStr Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
title_full_unstemmed Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
title_short Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
title_sort microfluidics and fluorescence microscopy protocol to study the response of c. elegans to chemosensory stimuli
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958070/
https://www.ncbi.nlm.nih.gov/pubmed/36853676
http://dx.doi.org/10.1016/j.xpro.2023.102121
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