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Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and im...
Autores principales: | Bruggeman, Christine W., Haasnoot, Guus H., Peterman, Erwin J.G. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958070/ https://www.ncbi.nlm.nih.gov/pubmed/36853676 http://dx.doi.org/10.1016/j.xpro.2023.102121 |
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