Annealing and purification of fluorescently labeled DNA substrates for in vitro assays

We present a protocol to generate high-quality fluorescently labeled DNA substrates that can be used for biochemical assays, including DNA-binding and nuclease activity assays. We describe polyacrylamide-gel-electrophoresis-based purification of DNA oligonucleotides, followed by annealing the oligon...

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Detalles Bibliográficos
Autores principales: Tse, Ying Wah Elizabeth, Yun, Hwa Young, Wyatt, Haley Doris Myskiw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958487/
https://www.ncbi.nlm.nih.gov/pubmed/36853679
http://dx.doi.org/10.1016/j.xpro.2023.102128

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958487/
https://www.ncbi.nlm.nih.gov/pubmed/36853679
http://dx.doi.org/10.1016/j.xpro.2023.102128

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