Cargando…

Annealing and purification of fluorescently labeled DNA substrates for in vitro assays

We present a protocol to generate high-quality fluorescently labeled DNA substrates that can be used for biochemical assays, including DNA-binding and nuclease activity assays. We describe polyacrylamide-gel-electrophoresis-based purification of DNA oligonucleotides, followed by annealing the oligon...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tse, Ying Wah Elizabeth, Yun, Hwa Young, Wyatt, Haley Doris Myskiw
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9958487/ https://www.ncbi.nlm.nih.gov/pubmed/36853679 http://dx.doi.org/10.1016/j.xpro.2023.102128

Ejemplares similares

Expression and purification of fused kinase from insect cells for in vitro kinase assay
por: Han, Yuhong, et al.
Publicado: (2021)

In Vitro Reconstitution and Imaging of Microtubule Dynamics by Fluorescence and Label-free Microscopy
por: Hirst, William Graham, et al.
Publicado: (2020)

Protocol for in vitro fluorescence assay of papain-like protease and cell-based immunofluorescence assay of coronavirus infection
por: Chang, JuOae, et al.
Publicado: (2023)

Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research
por: Jiang, Jian, et al.
Publicado: (2019)

Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts
por: Reusch, Sebastian, et al.
Publicado: (2020)

Protocol for the isolation and purification of human follicular dendritic cells for functional assays
por: Breeuwsma, Martijn, et al.
Publicado: (2023)

Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy
por: Ghoneim, Mohamed, et al.
Publicado: (2023)

Substrates, Plants, and Their Combinations for Water Purification of Urban Household Aquaponics Systems
por: Wu, Yi-Han, et al.
Publicado: (2022)

Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
por: El Maaiden, Ezzouhra, et al.
Publicado: (2019)

Purification of DNA repair protein complexes from mammalian cells
por: Liang, Chih-Chao, et al.
Publicado: (2021)

Comprehensive Interactome Mapping of the DNA Repair Scaffold SLX4 Using Proximity Labeling and Affinity Purification
por: Aprosoff, Camila M., et al.
Publicado: (2023)

Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv
por: Tsirkas, Ioannis, et al.
Publicado: (2023)

Protocol for in vitro phosphorylation of the MUS81-binding region of SLX4 using CDK1-cyclin B
por: Payliss, Brandon J., et al.
Publicado: (2023)

Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations
por: Gao, Yunrong, et al.
Publicado: (2021)

Evaluation of Jumonji C lysine demethylase substrate preference to guide identification of in vitro substrates
por: Hoekstra, Matthew, et al.
Publicado: (2022)

Detecting RNA-protein proximity at DNA double-strand breaks using combined fluorescence in situ hybridization with proximity ligation assay
por: Alagia, Adele, et al.
Publicado: (2023)

Generation of Inexpensive, Highly Labeled Probes for Fluorescence In Situ Hybridization (FISH)
por: Sharma, Rahul, et al.
Publicado: (2020)

Protocol for in vitro BCR-mediated plasma cell differentiation and purification of chromatin-associated proteins
por: Ochiai, Kyoko, et al.
Publicado: (2021)

Combined Fluorescence-Based in Vitro Assay for the Simultaneous Detection of Cell Viability and Alkaline Phosphatase Activity during Osteogenic Differentiation of Osteoblast Precursor Cells
por: Wilkesmann, Sebastian, et al.
Publicado: (2020)

Method of Microglial DNA-RNA Purification from a Single Brain of an Adult Mouse
por: Raihan, Md. Obayed, et al.
Publicado: (2021)

Whole-mount immunofluorescent labeling of the adult fly retina
por: Hsiao, Min-Chun, et al.
Publicado: (2022)

Rapid and Robust Continuous Purification of High-Titer Hepatitis B Virus for In Vitro and In Vivo Applications
por: Wettengel, Jochen M., et al.
Publicado: (2021)

FLAMBE: A kinetic fluorescence polarization assay to study activation of monomeric BAX
por: Mohammed, Jarvier N., et al.
Publicado: (2022)

Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay
por: Liu, Yan, et al.
Publicado: (2021)

Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
por: Giacomoni, Jessica, et al.
Publicado: (2023)

An In Vitro Microneutralization Assay for Influenza Virus Serology
por: Cuevas, Frans, et al.
Publicado: (2022)

Screening and in vitro antiviral assessment of small molecules against fluorescent protein-expressing Bunyamwera virus in a cell-based assay using high-content imaging
por: ter Horst, Sebastiaan, et al.
Publicado: (2021)

Preparation of site-specifically fluorophore-labeled polyubiquitin chains for FRET studies of Cdc48 substrate processing
por: Williams, Cameron, et al.
Publicado: (2023)

Isolation of adult mouse hippocampal neural stem cells for fluorescence loss in photobleaching assays
por: bin Imtiaz, Muhammad Khadeesh, et al.
Publicado: (2021)

Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach
por: Parui, Aasna L., et al.
Publicado: (2023)

Protocol to assess substrate dephosphorylation by serine/threonine phosphoprotein phosphatases in vitro
por: Wasserman, Jason S., et al.
Publicado: (2023)

Fluorescent pulse-chase labeling to monitor long-term mitochondrial degradation in primary hippocampal neurons
por: Schneider, Jordan R., et al.
Publicado: (2022)

Purification/annealing of graphene with 100-MeV Ag ion irradiation
por: Kumar, Sunil, et al.
Publicado: (2014)

Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock
por: Heisler, Joel, et al.
Publicado: (2019)

Purification of Recombinant Galectins Expressed in Bacteria
por: Prato, Cecilia Arahí, et al.
Publicado: (2020)

Protocol for High-Resolution Mapping of Splicing Products and Isoforms by RT-PCR Using Fluorescently Labeled Primers
por: Weathers, Indya, et al.
Publicado: (2020)

Protocol for in vitro lysine deacetylation to test putative substrates of class III deacetylases
por: Minten, Elizabeth V., et al.
Publicado: (2022)

A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
por: Fleitas, Andrea Luciana, et al.
Publicado: (2022)

Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis
por: Belan, Ondrej, et al.
Publicado: (2021)

Protein-oligonucleotide conjugates as model substrates for DNA-protein crosslink repair proteases
por: Reinking, Hannah K., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_9okl6pili7nt734qr8qoqv6kjt