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An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5

SIMPLE SUMMARY: For reliable detection of canine parainfluenza virus 5, a duplex real-time quantitative RT-PCR assay using the viral L gene and canine 16S rRNA primers and probe sets was developed in this study. The assay has high analytical sensitivity, specificity, and accuracy. Clinical evaluatio...

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Detalles Bibliográficos
Autores principales: Jeon, Gyu-Tae, Kim, Hye-Ryung, Shin, Yeun-Kyung, Kwon, Oh-Kyu, Kang, Hae-Eun, Kwon, Oh-Deog, Park, Choi-Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965950/
https://www.ncbi.nlm.nih.gov/pubmed/36851445
http://dx.doi.org/10.3390/vetsci10020142