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Conversion of Escherichia coli into Mixotrophic CO(2) Assimilation with Malate and Hydrogen Based on Recombinant Expression of 2-Oxoglutarate:Ferredoxin Oxidoreductase Using Adaptive Laboratory Evolution

We report the mixotrophic growth of Escherichia coli based on recombinant 2-oxoglutarate:ferredoxin oxidoreductase (OGOR) to assimilate CO(2) using malate as an auxiliary carbon source and hydrogen as an energy source. We employ a long-term (~184 days) two-stage adaptive evolution to convert heterot...

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Detalles Bibliográficos
Autores principales: Cheng, Yu-Chen, Huang, Wei-Han, Lo, Shou-Chen, Huang, Eugene, Chiang, En-Pei Isabel, Huang, Chieh-Chen, Yang, Ya-Tang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967407/
https://www.ncbi.nlm.nih.gov/pubmed/36838218
http://dx.doi.org/10.3390/microorganisms11020253