Cargando…

Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis

Reduction in cardiac contractility is common in severe sepsis. However, the pathological mechanism is still not fully understood. Recently it has been found that circulating histones released after extensive immune cell death play important roles in multiple organ injury and disfunction, particularl...

Descripción completa

Detalles Bibliográficos
Autores principales: Abrams, Simon T., Alhamdi, Yasir, Zi, Min, Guo, Fengmei, Du, Min, Wang, Guozheng, Cartwright, Elizabeth J., Toh, Cheng-Hock
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967552/
https://www.ncbi.nlm.nih.gov/pubmed/36834636
http://dx.doi.org/10.3390/ijms24043225
_version_ 1784897293158187008
author Abrams, Simon T.
Alhamdi, Yasir
Zi, Min
Guo, Fengmei
Du, Min
Wang, Guozheng
Cartwright, Elizabeth J.
Toh, Cheng-Hock
author_facet Abrams, Simon T.
Alhamdi, Yasir
Zi, Min
Guo, Fengmei
Du, Min
Wang, Guozheng
Cartwright, Elizabeth J.
Toh, Cheng-Hock
author_sort Abrams, Simon T.
collection PubMed
description Reduction in cardiac contractility is common in severe sepsis. However, the pathological mechanism is still not fully understood. Recently it has been found that circulating histones released after extensive immune cell death play important roles in multiple organ injury and disfunction, particularly in cardiomyocyte injury and contractility reduction. How extracellular histones cause cardiac contractility depression is still not fully clear. In this work, using cultured cardiomyocytes and a histone infusion mouse model, we demonstrate that clinically relevant histone concentrations cause significant increases in intracellular calcium concentrations with subsequent activation and enriched localization of calcium-dependent protein kinase C (PKC) α and βII into the myofilament fraction of cardiomyocytes in vitro and in vivo. Furthermore, histones induced dose-dependent phosphorylation of cardiac troponin I (cTnI) at the PKC-regulated phosphorylation residues (S43 and T144) in cultured cardiomyocytes, which was also confirmed in murine cardiomyocytes following intravenous histone injection. Specific inhibitors against PKCα and PKCβII revealed that histone-induced cTnI phosphorylation was mainly mediated by PKCα activation, but not PKCβII. Blocking PKCα also significantly abrogated histone-induced deterioration in peak shortening, duration and the velocity of shortening, and re-lengthening of cardiomyocyte contractility. These in vitro and in vivo findings collectively indicate a potential mechanism of histone-induced cardiomyocyte dysfunction driven by PKCα activation with subsequent enhanced phosphorylation of cTnI. These findings also indicate a potential mechanism of clinical cardiac dysfunction in sepsis and other critical illnesses with high levels of circulating histones, which holds the potential translational benefit to these patients by targeting circulating histones and downstream pathways.
format Online
Article
Text
id pubmed-9967552
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99675522023-02-27 Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis Abrams, Simon T. Alhamdi, Yasir Zi, Min Guo, Fengmei Du, Min Wang, Guozheng Cartwright, Elizabeth J. Toh, Cheng-Hock Int J Mol Sci Article Reduction in cardiac contractility is common in severe sepsis. However, the pathological mechanism is still not fully understood. Recently it has been found that circulating histones released after extensive immune cell death play important roles in multiple organ injury and disfunction, particularly in cardiomyocyte injury and contractility reduction. How extracellular histones cause cardiac contractility depression is still not fully clear. In this work, using cultured cardiomyocytes and a histone infusion mouse model, we demonstrate that clinically relevant histone concentrations cause significant increases in intracellular calcium concentrations with subsequent activation and enriched localization of calcium-dependent protein kinase C (PKC) α and βII into the myofilament fraction of cardiomyocytes in vitro and in vivo. Furthermore, histones induced dose-dependent phosphorylation of cardiac troponin I (cTnI) at the PKC-regulated phosphorylation residues (S43 and T144) in cultured cardiomyocytes, which was also confirmed in murine cardiomyocytes following intravenous histone injection. Specific inhibitors against PKCα and PKCβII revealed that histone-induced cTnI phosphorylation was mainly mediated by PKCα activation, but not PKCβII. Blocking PKCα also significantly abrogated histone-induced deterioration in peak shortening, duration and the velocity of shortening, and re-lengthening of cardiomyocyte contractility. These in vitro and in vivo findings collectively indicate a potential mechanism of histone-induced cardiomyocyte dysfunction driven by PKCα activation with subsequent enhanced phosphorylation of cTnI. These findings also indicate a potential mechanism of clinical cardiac dysfunction in sepsis and other critical illnesses with high levels of circulating histones, which holds the potential translational benefit to these patients by targeting circulating histones and downstream pathways. MDPI 2023-02-06 /pmc/articles/PMC9967552/ /pubmed/36834636 http://dx.doi.org/10.3390/ijms24043225 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abrams, Simon T.
Alhamdi, Yasir
Zi, Min
Guo, Fengmei
Du, Min
Wang, Guozheng
Cartwright, Elizabeth J.
Toh, Cheng-Hock
Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis
title Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis
title_full Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis
title_fullStr Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis
title_full_unstemmed Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis
title_short Extracellular Histone-Induced Protein Kinase C Alpha Activation and Troponin Phosphorylation Is a Potential Mechanism of Cardiac Contractility Depression in Sepsis
title_sort extracellular histone-induced protein kinase c alpha activation and troponin phosphorylation is a potential mechanism of cardiac contractility depression in sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967552/
https://www.ncbi.nlm.nih.gov/pubmed/36834636
http://dx.doi.org/10.3390/ijms24043225
work_keys_str_mv AT abramssimont extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis
AT alhamdiyasir extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis
AT zimin extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis
AT guofengmei extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis
AT dumin extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis
AT wangguozheng extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis
AT cartwrightelizabethj extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis
AT tohchenghock extracellularhistoneinducedproteinkinasecalphaactivationandtroponinphosphorylationisapotentialmechanismofcardiaccontractilitydepressioninsepsis