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Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model

We investigated the role of a sirolimus-embedded silk microneedle (MN) wrap as an external vascular device for drug delivery efficacy, inhibition of neointimal hyperplasia, and vascular remodeling. Using dogs, a vein graft model was developed to interpose the carotid or femoral artery with the jugul...

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Autores principales: Kim, Jung-Hwan, Jang, Eui Hwa, Ryu, Ji-Yeon, Lee, Jiyong, Kim, Jae Ho, Ryu, Wonhyoung, Youn, Young-Nam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967879/
https://www.ncbi.nlm.nih.gov/pubmed/36834717
http://dx.doi.org/10.3390/ijms24043306
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author Kim, Jung-Hwan
Jang, Eui Hwa
Ryu, Ji-Yeon
Lee, Jiyong
Kim, Jae Ho
Ryu, Wonhyoung
Youn, Young-Nam
author_facet Kim, Jung-Hwan
Jang, Eui Hwa
Ryu, Ji-Yeon
Lee, Jiyong
Kim, Jae Ho
Ryu, Wonhyoung
Youn, Young-Nam
author_sort Kim, Jung-Hwan
collection PubMed
description We investigated the role of a sirolimus-embedded silk microneedle (MN) wrap as an external vascular device for drug delivery efficacy, inhibition of neointimal hyperplasia, and vascular remodeling. Using dogs, a vein graft model was developed to interpose the carotid or femoral artery with the jugular or femoral vein. The control group contained four dogs with only interposed grafts; the intervention group contained four dogs with vein grafts in which sirolimus-embedded silk-MN wraps were applied. After 12-weeks post-implantation, 15 vein grafts in each group were explanted and analyzed. Vein grafts applied with the rhodamine B–embedded silk-MN wrap showed far higher fluorescent signals than those without the wrap. The diameter of vein grafts in the intervention group decreased or remained stable without dilatation; however, it increased in the control group. The intervention group had femoral vein grafts with a significantly lower mean neointima-to-media ratio, and had vein grafts with an intima layer showing a significantly lower collagen density ratio than the control group. In conclusion, sirolimus-embedded silk-MN wrap in a vein graft model successfully delivered the drug to the intimal layer of the vein grafts. It prevented vein graft dilatation, avoiding shear stress and decreasing wall tension, and it inhibited neointimal hyperplasia.
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spelling pubmed-99678792023-02-27 Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model Kim, Jung-Hwan Jang, Eui Hwa Ryu, Ji-Yeon Lee, Jiyong Kim, Jae Ho Ryu, Wonhyoung Youn, Young-Nam Int J Mol Sci Article We investigated the role of a sirolimus-embedded silk microneedle (MN) wrap as an external vascular device for drug delivery efficacy, inhibition of neointimal hyperplasia, and vascular remodeling. Using dogs, a vein graft model was developed to interpose the carotid or femoral artery with the jugular or femoral vein. The control group contained four dogs with only interposed grafts; the intervention group contained four dogs with vein grafts in which sirolimus-embedded silk-MN wraps were applied. After 12-weeks post-implantation, 15 vein grafts in each group were explanted and analyzed. Vein grafts applied with the rhodamine B–embedded silk-MN wrap showed far higher fluorescent signals than those without the wrap. The diameter of vein grafts in the intervention group decreased or remained stable without dilatation; however, it increased in the control group. The intervention group had femoral vein grafts with a significantly lower mean neointima-to-media ratio, and had vein grafts with an intima layer showing a significantly lower collagen density ratio than the control group. In conclusion, sirolimus-embedded silk-MN wrap in a vein graft model successfully delivered the drug to the intimal layer of the vein grafts. It prevented vein graft dilatation, avoiding shear stress and decreasing wall tension, and it inhibited neointimal hyperplasia. MDPI 2023-02-07 /pmc/articles/PMC9967879/ /pubmed/36834717 http://dx.doi.org/10.3390/ijms24043306 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jung-Hwan
Jang, Eui Hwa
Ryu, Ji-Yeon
Lee, Jiyong
Kim, Jae Ho
Ryu, Wonhyoung
Youn, Young-Nam
Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model
title Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model
title_full Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model
title_fullStr Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model
title_full_unstemmed Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model
title_short Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model
title_sort sirolimus-embedded silk microneedle wrap to prevent neointimal hyperplasia in vein graft model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967879/
https://www.ncbi.nlm.nih.gov/pubmed/36834717
http://dx.doi.org/10.3390/ijms24043306
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