A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals

Crystallizing G protein-coupled receptors (GPCRs) in lipidic cubic phase (LCP) often yields crystals suited for the cryogenic electron microscopy (cryoEM) method microcrystal electron diffraction (MicroED). However, sample preparation is challenging. Embedded crystals cannot be targeted topologicall...

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Detalles Bibliográficos
Autores principales: Martynowycz, Michael W., Shiriaeva, Anna, Clabbers, Max T. B., Nicolas, William J., Weaver, Sara J., Hattne, Johan, Gonen, Tamir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968316/
https://www.ncbi.nlm.nih.gov/pubmed/36841804
http://dx.doi.org/10.1038/s41467-023-36733-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968316/
https://www.ncbi.nlm.nih.gov/pubmed/36841804
http://dx.doi.org/10.1038/s41467-023-36733-4

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