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Capture, Release, and Identification of Newly Synthesized Proteins for Improved Profiling of Functional Translatomes

New protein synthesis is regulated both at the level of mRNA transcription and translation. RNA-Seq is effective at measuring levels of mRNA expression, but techniques to monitor mRNA translation are much more limited. Previously, we reported results from O-propargyl-puromycin (OPP) labeling of prot...

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Detalles Bibliográficos
Autores principales: Phillips, Nancy J., Vinaithirthan, Bala M., Oses-Prieto, Juan A., Chalkley, Robert J., Burlingame, Alma L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971285/
https://www.ncbi.nlm.nih.gov/pubmed/36642223
http://dx.doi.org/10.1016/j.mcpro.2023.100497