tRNAs Are Stable After All: Pitfalls in Quantification of tRNA from Starved Escherichia coli Cultures Exposed by Validation of RNA Purification Methods

tRNAs and ribosomal RNAs are often considered stable RNAs. In contrast to this view, we recently proposed that tRNAs are degraded during amino acid starvation and drug-induced transcription inhibition. However, reevaluation of our experimental approach revealed that common RNA extraction methods suf...

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Detalles Bibliográficos
Autores principales: Prossliner, Thomas, Agrawal, Shreya, Heidemann, Ditte F., Sørensen, Michael A., Svenningsen, Sine L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9973347/
https://www.ncbi.nlm.nih.gov/pubmed/36598190
http://dx.doi.org/10.1128/mbio.02805-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9973347/
https://www.ncbi.nlm.nih.gov/pubmed/36598190
http://dx.doi.org/10.1128/mbio.02805-22

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