Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP

The dorsal raphe nucleus (DR) and the pre-Bötzinger complex (PBC) may play an important role in regulating seizure-induced respiratory arrest (S-IRA), the main contributor to sudden unexpected death in epilepsy. Here, we describe pharmacological, optogenetic, and retrograde labeling approaches to sp...

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Autores principales: Wang, YuLing, Xu, Qing, Yu, Qian, Gu, LeYuan, Ma, HaiXiang, Shen, Yue, Lian, XiTing, Shao, WeiHui, Gu, JiaXuan, Liu, Lu, Zhang, HongHai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975696/
https://www.ncbi.nlm.nih.gov/pubmed/36861823
http://dx.doi.org/10.1016/j.xpro.2023.102129
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author Wang, YuLing
Xu, Qing
Yu, Qian
Gu, LeYuan
Ma, HaiXiang
Shen, Yue
Lian, XiTing
Shao, WeiHui
Gu, JiaXuan
Liu, Lu
Zhang, HongHai
author_facet Wang, YuLing
Xu, Qing
Yu, Qian
Gu, LeYuan
Ma, HaiXiang
Shen, Yue
Lian, XiTing
Shao, WeiHui
Gu, JiaXuan
Liu, Lu
Zhang, HongHai
author_sort Wang, YuLing
collection PubMed
description The dorsal raphe nucleus (DR) and the pre-Bötzinger complex (PBC) may play an important role in regulating seizure-induced respiratory arrest (S-IRA), the main contributor to sudden unexpected death in epilepsy. Here, we describe pharmacological, optogenetic, and retrograde labeling approaches to specifically modulate the DR to PBC serotonergic pathway. We detail steps for implanting optical fibers and viral infusion into DR and PBC regions and optogenetic techniques for exploring the role of 5-hydroxytryptophan (5-HT) neural circuit of DR-PBC in S-IRA. For complete details on the use and execution of this protocol, please refer to Ma et al. (2022).(1)
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spelling pubmed-99756962023-03-02 Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP Wang, YuLing Xu, Qing Yu, Qian Gu, LeYuan Ma, HaiXiang Shen, Yue Lian, XiTing Shao, WeiHui Gu, JiaXuan Liu, Lu Zhang, HongHai STAR Protoc Protocol The dorsal raphe nucleus (DR) and the pre-Bötzinger complex (PBC) may play an important role in regulating seizure-induced respiratory arrest (S-IRA), the main contributor to sudden unexpected death in epilepsy. Here, we describe pharmacological, optogenetic, and retrograde labeling approaches to specifically modulate the DR to PBC serotonergic pathway. We detail steps for implanting optical fibers and viral infusion into DR and PBC regions and optogenetic techniques for exploring the role of 5-hydroxytryptophan (5-HT) neural circuit of DR-PBC in S-IRA. For complete details on the use and execution of this protocol, please refer to Ma et al. (2022).(1) Elsevier 2023-02-20 /pmc/articles/PMC9975696/ /pubmed/36861823 http://dx.doi.org/10.1016/j.xpro.2023.102129 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wang, YuLing
Xu, Qing
Yu, Qian
Gu, LeYuan
Ma, HaiXiang
Shen, Yue
Lian, XiTing
Shao, WeiHui
Gu, JiaXuan
Liu, Lu
Zhang, HongHai
Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP
title Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP
title_full Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP
title_fullStr Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP
title_full_unstemmed Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP
title_short Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP
title_sort protocol for modulation of the serotonergic dr-pbc neural circuit to prevent sudep in the acoustic and ptz-induced dba/1 mouse models of sudep
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975696/
https://www.ncbi.nlm.nih.gov/pubmed/36861823
http://dx.doi.org/10.1016/j.xpro.2023.102129
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